Pgem T Easy Vector. The insertion site is flanked by BstZI, EcoRI, and NotI sites. Promega pGEM™-T and pGEM™-T Easy Vector Systems.
Convenient systems for the routine subcloning of PCR products. pGEM-T Easy Vector Systems. Parental vector for TA cloning of PCR products. The pGEM®-T Easy Vector multiple cloning region is flanked by recognition sites for the restriction enzymes EcoRI, BstZI and NotI, thus providing three single-enzyme digestions for release of the insert.
This method takes advantage of the "A" overhang added by a PCR enzyme like Taq DNA Polymerase.
The pGEM -T Easy Vector multiple cloning region is flanked by recognition sites for the restriction enzymes EcoRI, BstZI and NotI, providing three The MCS of the pGEM-T Easy Vector contains sequences on either side of the insert that are recognized by the restriction enzymes Not I and EcoR I.
The insertion site is flanked by BstZI, EcoRI, and NotI sites. Convenient systems for the routine subcloning of PCR products. pGEM-T Easy Vector Systems. That arrow is usually an agent like an insect or rodent, but it can also be inanimate.